/> ED I T O R S C H O I C E IN N O V A T IO N A W A R D
X

Building a microscopic biofuel factory (photos)

<p>Start-up Joule Unlimited is at the forefront of a wave of researchers and companies using genetic engineering to make biofuels.</p>

headshots_martin_laMonica_140x100.jpg
Martin LaMonica
petri.jpg
1 of 8 Martin LaMonica/CNET

Growing colonies

CAMBRIDGE, Mass.--Joule Unlimited is one of many companies using gene databases and genetic engineering to design a better system for making biofuels. Here the senior vice president of biological services, Dan Robertson, shows off a petri dish where employees grow different strains of a cyanobacteria Joule Unlimited has engineered to make diesel fuel.

Joule_Copy_of_Pilot_site_3.jpg
2 of 8 Joule Unlimited

Demo plant

Designing a microbe is only half of Joule's work. The other is making bioreactors, such as this one now being tested at a facility in Texas. The cyanobacteria are designed to secrete a fuel, which naturally separates from the solution it grows in and is extracted from the top of the bioreactor. Water and carbon dioxide from a power plant or wastewater facility is pumped in from the bottom and circulated through the other bioreactors. Scaling up should be straightforward because it simply means building more of the same types of reactors.

colonies.jpg
3 of 8 Martin LaMonica/CNET

Gene therapy

Growing a colony of a particular strain can take about a day, but molecular biologists are trying hundreds of different gene combinations. About 80 percent of a biologist's time is spent on the computer, identifying genes and designing lab tests.

bubbles.jpg
4 of 8 Martin LaMonica/CNET

Tweaking conditions

As part of its testing, Joule Unlimited purchased specialized equipment that allows biologists to get fine-grained control over the environmental conditions for growing microbes. Here carbon dioxide and nitrogen, the main element in air, is pumped in from the bottom to stimulate growth of the cyanobacteria. Red LEDs (seen a bit at bottom of photo) are the source for mimicking sunlight.

biotesting.jpg
5 of 8 Martin LaMonica/CNET

Metabolic engineering

With these machines, biologists can mimic the light that they can anticipate in different regions over the course of the day or during different seasons. They can also test how factors such as pH and oxygen levels can affect growth rates.

flask.jpg
6 of 8 Martin LaMonica/CNET

Incubator

An incubator for growing sample strains allows biologists to control different environmental factors and measure growth rates.

metabolic_pathways.jpg
7 of 8 Martin LaMonica/CNET

Metabolic engineering

Joule Unlimited is focused on metabolic engineering, or manipulating the functions of organisms, in this case to make fuel. This poster appears in a few spots at the company's lab. An organism has a number of biological pathways to perform certain functions, such as regulating metabolism for changing environmental conditions or multiplying. Through its engineering, Joule Unlimited is controlling those metabolic pathways so that organisms will secrete hydrocarbon molecules which can be used for liquid fuel or other chemicals.

lifesciencsq.jpg
8 of 8 Martin LaMonica/CNET

Biotech avenue

Joule Unlimited is located in Kendall Square, Cambridge, among a stretch of older buildings dubbed "Life Sciences Square" in the neighborhood of many pharmaceutical companies. Having an infrastructure, such as third-party companies to do DNA sequencing, around genetic engineering helps the company rapidly identify promising strains.

More Galleries

2022's best TV shows you can't miss on Netflix, HBO, Disney Plus and more

More Galleries

2022's best TV shows you can't miss on Netflix, HBO, Disney Plus and more

61 Photos
Nintendo Switch: The best games to play right now

More Galleries

Nintendo Switch: The best games to play right now

41 Photos
The Porsche Taycan Sport Turismo is the EV of our dreams

More Galleries

The Porsche Taycan Sport Turismo is the EV of our dreams

7 Photos
Movies coming in 2022 from Marvel, Netflix, DC and more

More Galleries

Movies coming in 2022 from Marvel, Netflix, DC and more

65 Photos
Subaru Solterra STI feels like a natural extension

More Galleries

Subaru Solterra STI feels like a natural extension

5 Photos
2022 Porsche Cayenne adds snazzy Platinum Edition

More Galleries

2022 Porsche Cayenne adds snazzy Platinum Edition

9 Photos
Best dating apps of 2021

More Galleries

Best dating apps of 2021

13 Photos