Probably won't help. Simple fact is, USB ports aren't that durable. They go bad all the time, and while they don't usually all go bad at once, it's not out of the question.
Installing Windows 7 RC will likely not do a thing to resolve this, as it's likely a hardware issue. The only thing that will fix it is a motherboard replacement.
My sister has a and old computer (Pentium 4HT @2.4 ghz, Asus p4p800vm motherboard,Nvidia geforce 6800, etc) and we instated windows vista home premium on it about 6 months agao. But just recently we started experiencing USB problems, meaning no matter what device on what port vista wont see the device. Then when i open the device mangier and scroll down to usb it has an exclamation point with "unknown device" next to it. When i plug a device in on any other computer it makes the "duh ding" noise but on her computer i hear the "device disconected" noise played on top of the "device connected" noise! I dont know what else to do. ive allready tried tried to update the usb main driver in the device mangier but it said that the best driver was already instaled. should i just scrap Vista and instal Windows 7 RC? please help!
-Brian

Chowhound
Comic Vine
GameFAQs
GameSpot
Giant Bomb
TechRepublic