The Prefetch Folder is supposed to speed up the operation of XP. It stores files from apps that you use frequently so that XP does not have to seek for them each time.
Deleting all files in this folder means XP will have to replace those that are needed the next time you boot up. This could be why it may take a little longer to boot up.
But once they are replaced the next boot should be back to normal.
I'd leave the layout folder alone.
Don't fix what ain't broke.
I read here a while back you could delete all the files in the windows folder prefetch and xp would rebuild them and this was good to do every so often. But I read somewhere on the net that this could cause restart problems and not to do it. Has anybody here done this and what was your opinion. Also there is a file under prefetch call Layout that is different from the rest. Do I delete this file also, it looks important and am afraid to delete it. I am running xp on a athlon 1.6 and 512 ram

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