try a system restore to yesterday when they were working. If that fails, check your CMOS setup to confirm that USB function is still enabled. If that's okay, look in Device Manager for the USB controller. Any warning icon? R-click and select properties for a message on its status. If that appears okay, check the USB port drivers listed in Device Manager as Root Hubs. R-click and select properties. What's the status?
I woke up today to use my laptop computer and for some reason none of the USB's are working. As a result I cant use my printer, mouse etc.
It is a brand new Acer with Windows XP (not sure model number, ill look and post later)