There is no limit for the number of folders in a subfolder. And no limit to the number of files in a subfolder. Until you run out of disk space.
But worth noting is the OS slows a little with every 250 or so entries in the directory. Eventually it can take an hour to open a file.
There is another issue which is not a limit of the filesystem, but of Explorer. With this DOS based OS, the 64 thousand byte resource pool can be depleted and Explorer may fail.
Gateway 400 Select PC; Windows 98SE; IE6 SP2; 160MB ram
Is there a maximum # of subfolders I can put into a folder?
Is there a maximum # of files I can put into a sub-folder?
E.G. I have My Pictures folder full of 50 subfolders on various subjects. Within each subfolder, I have many files (.jpg and .gif files).
Is there a limit on filling these folders and subfolders?