Probably not. You should be able to format your boot drive, without having to worry about the others. HOWEVER, you will likely have to reinstall every app installed on the other drive(s).
C: 74GB WD Raptor
D: 640GB Seagate
E: 1TB WD External
Currently, these are all NTFS.
On the C: is my install of Vista Ultimate 64-bit and a few other programs I use.
On the D: is my storage of movies, music, bulk programs, and games.
On the E: my storage of HD content and where I had my C: and D: making continuous back-ups to.
Last month, I noticed the back-ups failed and got the message that my C: was corrupted, constant BSOD, as well as many programs failing for various strange reasons. This has left me with just formatting the drives, but my question is, can I just format C:? Does D: need it as well? I'm fine losing C: is there a separate way I can back up D: and go for the double format?