Why not try it?
Windows 2000 isn't so different from NT4.0, so it might work. But just as well, the installation programs checks supported OS'es, doesn't know NT4.0 and gives an error message.
You'll only know when you try.
I have this greeting card program that will run in Windows 95,98 and NT 4.0. I would like to know if it will run in Windows 2000. Sierra Home has since gone out of business and Hallmark Software did not publish the p[rogram>