What exactly do you mean with "ISO files". That are strange things to have on a USB stick.
The .iso file is an image of a DVD, more or less. You should use Microsofts Media Creation Tool to put the files on that image on a USB-stick and make it bootable. Did you do that?
Where you made that USB-stick is totally irrelevant. But you need to run the install on the target laptop.
If I have the operating system ISO files on a usb drive, and a extra hard drive. I can remove the hard drive from my laptop install the extra HD then boot from the usb to install the operating system to that hard drive. But will it run on that laptop if the ISO file was created on said laptop?