An ISO is just a copy of a cd, so if the cd it's a copy of plays, a new copy of the ISO to a new cd will play also.
There are exceptions with old players that can't read burned CD's, but that's the only issue you can encounter. Why not try?
Hi there, I'm relatively new to this sort of stuff so forgive me for my ignorance, but I was wondering if you made an ISO with say, all the music files in mp3 or WAV format contained within, then burned it to a CDR, would that work in a CD player after burning it? If not is there a similar way to achieve the same goals? I'm trying to use a hard drive to keep a library of CDs because I burn A LOT in my cd duplicator, but being able to keep a digital library would be better than keeping track of a load of master CDs