The title is based on the 2014-2015 Marvel Comics Spider-Verse event, which saw just about every spider-powered character breaching their dimensional barriers to battle Morlun and the Inheritors, a group of beings intent on slaughtering them all.
The movie doesn't stick to that story line beyond the interdimensional aspect, but let's dive into some of the characters' histories and see how the movie celebrates Spidey's 56-year history.
Be warned, this contains full, hard-core spoilers.
Peter Parker -- 1 and 2
We don't need to explain this guy, right? Bitten by a radioactive spider, his uncle shot to death, "with great power there must also come great responsibility" -- you know this character.
The very first Peter we meet in the movie is played by Chris Pine (James T. Kirk in the rebooted Star Trek movies), but he's killed by Kingpin shortly after teenager Mile Morales gets bitten by a genetically modified spider.
This version of the character is a bit of a superstar, beloved by the people of New York and married to Mary Jane (Zoe Kravitz). He's also blond, perhaps as a nod to the comics' Ben Reilly -- Peter's clone who took over as Spidey for a brief spell in the '90s infamous Clone Saga. Ben dyed his hair to differentiate himself from Peter in public and designed a cool new costume to do so in his superhero life, in 1996's Sensational Spider-Man No. 0.
The movie's main Peter is played by Jake Johnson (of New Girl fame) and acts as a mentor to Miles after being dragged into the teen's universe.
"It's Peter Parker at 40, it's Peter Parker with a bad back... and who's not sure if he still wants to be Spider-Man, and what it means to be Spider-Man after the excitement of being Spider-Man," Johnson said during the 2018 San Diego Comic-Con panel.
"That just got really heavy, folks. But that's Peter Parker. It's chubby Peter Parker."
This Brooklyn teen (played by Shameik Moore) gets spider powers but is left mentorless after the first Peter's death. It's a little different for Miles' comic counterpart, who first appeared in Marvel's Ultimate universe -- an alternate continuity to its main 616 universe. The Ultimate version of Peter appeared to die in Ultimate Comics Spider-Man No. 160. (He later came back because comics are weird like that.)
Miles was created by writer Brian Michael Bendis and artist Sara Pichelli and has had his own comic series pretty consistently since his debut in Ultimate Fallout No. 4. After Bendis departed Marvel for DC in May, Miles' solo book took a break.
However, it returned on Dec. 12, with writer Saladin Ahmed and artist Javier Garrón on creative duties, two days before Miles made his movie debut.
There's also comic book precedent for Miles running into an older Peter from another universe. The pair first met in the 2012 Spider-Men miniseries, which sees them taking on Mysterio.
"He's a black Spider-Man -- black and Mexican. I think that that's very powerful and iconic," Moore said of the character.
This character comes from a universe where Gwen Stacy, instead of Peter, was bitten by a radioactive spider. In the main Marvel universe, Gwen is best known for her death at the hands of Green Goblin in Amazing Spider-Man No. 121 -- a watershed moment in superhero comics.
Fortunately, the movie's version of Gwen (voiced by Hailee Steinfeld) avoided that fate and took on the name Spider-Woman. Her comic book adventures have seen her battling familiar characters with neat twists, like a corrupt version of Matt Murdock/Daredevil.
Created by writer Jason Latour and artist Robbi Rodriguez, she first appeared in 2014's Edge of Spider-Verse No. 2 and her striking design won her an ongoing Spider-Gwen series, but that ended after its 34th issue.
"I get to play a female superhero, which was pretty cool -- and not just any female superhero, but the first Spider-Woman," said Steinfeld. "That was so exciting to me, and the fact that she really is the toughest and the coolest and the smartest and the most capable one in the room, and she knows it."
Gwen isn't actually the first Spider-Woman (that honor goes to Jessica Drew, who first showed up in 1977's Marvel Spotlight No. 32), but is the first to appear in a movie.
How to watch every Marvel Cinematic Universe film in the right order
This Peter Parker of an alternate timeline is quite unlike the hero we're used to, since he's from 1933 and battles the gangsters of the Great Depression.
1930s Peter was created by writers David Hine and Fabrice Sapolsky, along with artist Carmine Di Giandomenico. He made his first appearance in 2009's Spider-Man Noir No. 1. Unlike the other characters we've looked at, he hasn't had an ongoing series. His story was told in a self-titled miniseries and its sequel, Eyes Without a Face. Both lasted four issues.
Comedian John Mulaney plays porktacular Spider-Ham, the most ridiculous member of the crew.
Peter Porker is a parody character created by writer Tom DeFalco and artist Mark Armstrong. He made his first appearance in 1983's Marvel Tails Starring Peter Porker, the Spectacular Spider-Ham No. 1.
This was followed by a 17-issue series, which saw dozens of other anthropomorphic versions of familiar characters showing up with amazing names including Ducktor Doom, Captain Americat (Steve Mouser) and Goose Rider.
Since his series ended, Spider-Ham has shown up in wacky guest appearances through the years.
Peni Parker, created by writer Gerard Way (formerly the singer in My Chemical Romance) and artist Jake Wyatt, is the 14-year-old daughter of the SP//dr mech suit's original pilot.
After her father was killed in action, she took his place by letting the radioactive spider that formed the other half of the suit's CPU bite her -- forming a psychic link.
This crime boss was created by writer Stan Lee and artist John Romita Sr. for his first comic book appearance in 1967's Amazing Spider-Man No. 50. Kingpin has been a recurring villain ever since, but the current Marvel Comics status quo has him as New York City's democratically elected mayor.
In the movie, a flashback shows that Fisk's wife Vanessa (Lake Bell) and son Richard fled when they happened upon him attacking Spidey, only to be killed by a truck as they left. He's determined to replace them with their counterparts from another universe, at any cost.
Vanessa Fisk first appeared in Amazing Spider-Man No. 70 back in 1969, while Richard first appeared in the 83rd issue of the same series in 1970. He went on to become a crime lord himself, going by the lame Schemer and more refined Rose.
Fisk is backed up in the movie by the purple-hooded Prowler. The first person to take on this identity was Hobie Brown, in 1969's Amazing Spider-Man No. 78, and he became an ally of Spidey.
However, this Prowler is based on the Ultimate universe version, where the mask is worn by Miles' morally questionable and antagonist uncle, Aaron Davis. He first appeared in 2011's Ultimate Comics: Spider-Man No. 1, and Mahershala Ali plays him the movie.
Doctor Octopus is one of the movie's surprise villains. Olivia Octavius (Kathryn Hahn) is a new version of the classic Spidey villain (who first showed up in Amazing Spider-Man No. 3) working for Fisk.
However, she's not the first female Doc Ock we've ever seen -- that honor goes to Carolyn Trainer, who first appeared in Amazing Spider-Man No. 406 in 1995 and took over for the original while he was dead. She later played a role in his resurrection, because comics.
Peter also faces off against demonic version of the Green Goblin, Spider-Man's greatest villain, at the start of the movie. The original costumed Goblin first showed up in 1964's Amazing Spider-Man No. 14, but the monstrous version's first appearance was in Ultimate Spider-Man No. 4.
Both versions are Norman Osborn, the father of Peter's best friend Harry, driven to insanity by his experiments in a quest to gain superpowers.